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ion ampliseq multiplex pcr primer panel  (Thermo Fisher)


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    Thermo Fisher ion ampliseq multiplex pcr primer panel
    Ion Ampliseq Multiplex Pcr Primer Panel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ion ampliseq multiplex pcr primer panel/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    ion ampliseq multiplex pcr primer panel - by Bioz Stars, 2026-06
    90/100 stars

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    The procedure of <t>target</t> <t>SNP-seq</t> in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of <t>PCR.</t> The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.
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    Image Search Results


    Coverage evenness of SARS‐CoV‐2 genome of multiplex PCR mix panels. (A) Coverage depth profile of six clinical samples for the 1K‐panel (~1000‐bp amplicons) and the ARTIC V3 primer panel (~400‐bp amplicons). Depth is in standard logarithmic scale, with 100‐fold denoted by the dashed line. Bold black lines denote median depth, and grey shadows indicate the minimum and maximum depths. Mean depth is defined as the average site depth on the targeted viral genome (excluding the 3′ and 5′ ends). (B) Two thresholds, >20% and >30% mean depth, were used to define regions of high coverage. Viral titers were quantified by the qRT‐PCR C t value of SARS‐CoV‐2 detection. (C) The correlation between coverage evenness and viral titers of samples for the 1K‐panel. The proportions of genome regions with a >20% mean depth for 49 clinical samples. SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2

    Journal: Journal of Medical Virology

    Article Title: Assessment of two‐pool multiplex long‐amplicon nanopore sequencing of SARS‐CoV‐2

    doi: 10.1002/jmv.27336

    Figure Lengend Snippet: Coverage evenness of SARS‐CoV‐2 genome of multiplex PCR mix panels. (A) Coverage depth profile of six clinical samples for the 1K‐panel (~1000‐bp amplicons) and the ARTIC V3 primer panel (~400‐bp amplicons). Depth is in standard logarithmic scale, with 100‐fold denoted by the dashed line. Bold black lines denote median depth, and grey shadows indicate the minimum and maximum depths. Mean depth is defined as the average site depth on the targeted viral genome (excluding the 3′ and 5′ ends). (B) Two thresholds, >20% and >30% mean depth, were used to define regions of high coverage. Viral titers were quantified by the qRT‐PCR C t value of SARS‐CoV‐2 detection. (C) The correlation between coverage evenness and viral titers of samples for the 1K‐panel. The proportions of genome regions with a >20% mean depth for 49 clinical samples. SARS‐CoV‐2, severe acute respiratory syndrome coronavirus 2

    Article Snippet: The generated first‐strand cDNA was used as the template for SARS‐CoV‐2‐specific amplification with different multiplex PCR primer panels and NEBNext High‐Fidelity 2X PCR Master Mix (New England Biolabs) following the manufacturer's instructions.

    Techniques: Multiplex Assay, Quantitative RT-PCR

    The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.

    Journal: Scientific Reports

    Article Title: A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties

    doi: 10.1038/s41598-020-62518-6

    Figure Lengend Snippet: The procedure of target SNP-seq in genotyping 261 varieties with 163 perfect SNP. Library construction of target SNP-seq included two round of PCR. The first round of PCR was to capture the target SNP locus through multiplex PCR. The second round of PCR aimed to add a unique barcode adaptor for each DNA sample. Finally, the constructed library was sequenced on Highseq X platform.

    Article Snippet: Finally, all perfect SNP loci were sent to the Molbreeding Biotechnology Company (Shijiazhuang, China) for multiplex PCR primer panel design.

    Techniques: Multiplex Assay, Construct